Identification of Antinorovirus Genes in Human Cells Using Genome-Wide CRISPR Activation Screening.

ABSTRACT: Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide, yet host factors that restrict NoV replication are not well understood. Here, we use a CRISPR activation genome-wide screening to identify host genes that can inhibit murine norovirus (MNoV) replication in human cells. Our screens identified with high confidence 49 genes that can inhibit MNoV infection when overexpressed. A significant number of these genes are in interferon and immune regulation signaling networks, but surprisingly, the majority of the genes identified are neither associated with innate or adaptive immunity nor associated with any antiviral activity. Confirmatory studies of eight of the genes validate the initial screening data. Mechanistic studies on TRIM7 demonstrated a conserved role of the mol

SUBMITTER: Orchard RC 

PROVIDER: S-EPMC6288323 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature