Low-Bias RNA Sequencing of the HIV-2 Genome from Blood Plasma.

ABSTRACT: Accurate determination of the genetic diversity present in the HIV quasispecies is critical for the development of a preventative vaccine: in particular, little is known about viral genetic diversity for the second type of HIV, HIV-2. A better understanding of HIV-2 biology is relevant to the HIV vaccine field because a substantial proportion of infected people experience long-term viral control, and prior HIV-2 infection has been associated with slower HIV-1 disease progression in coinfected subjects. The majority of traditional and next-generation sequencing methods have relied on target amplification prior to sequencing, introducing biases that may obscure the true signals of diversity in the viral population. Additionally, target enrichment through PCR requires a priori sequence

SUBMITTER: James KL 

PROVIDER: S-EPMC6288329 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature