Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles.

ABSTRACT: The HIV-1 envelope (Env) glycans shield the surface of Env from the immune system and form integral interactions important for a functional Env. To understand how individual N-glycosylation sites (NGS) coordinate to form a dynamic shield and evade the immune system through mutations, we tracked 20 NGS in Env from HIV-transmitted/founder (T/F) and immune escape variants and their mutants involving the N262 glycan. NGS were profiled in a site-specific manner using a high-resolution mass spectrometry (MS)-based workflow. Using this site-specific quantitative heterogeneity profiling, we empirically characterized the interdependent NGS of a microdomain in the high-mannose patch (HMP). The changes (shifts) in NGS heterogeneity between the T/F and immune escape variants defined a range of NGS tha

SUBMITTER: Hargett AA 

PROVIDER: S-EPMC6288332 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature