Surface Immunoproteomics Reveals Potential Biomarkers in Alicyclobacillus acidoterrestris.

ABSTRACT: Alicyclobacillus acidoterrestris is a major putrefying bacterium that can cause pecuniary losses in the global juice industry. Current detection approaches are time-consuming and exhibit reduced specificity and sensitivity. In this study, an immunoproteomic approach was utilized to identify specific biomarkers from A. acidoterrestris for the development of new detection methods. Cell surface-associated proteins were extracted and separated by 2-D (two-dimensional) gel electrophoresis. Immunogenic proteins were detected by Western blot analysis using antisera against A. acidoterrestris. Twenty-two protein spots exhibiting immunogenicity were excised and eighteen of the associated spots were successfully identified by matrix-assisted laser desorption/ionization time-of-f

SUBMITTER: Shi Y 

PROVIDER: S-EPMC6288362 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature