Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea.

ABSTRACT: Lack of sensory hair cell (HC) regeneration in mammalian adults is a major contributor to hearing loss. In contrast, the neonatal mouse cochlea retains a transient capacity for regeneration, and forced Wnt activation in neonatal stages promotes supporting cell (SC) proliferation and induction of ectopic HCs. We currently know little about the temporal pattern and underlying mechanism of this age-dependent regenerative response. Using an in vitro model, we show that Wnt activation promotes SC proliferation following birth, but prior to postnatal day (P) 5. This age-dependent decline in proliferation occurs despite evidence that the Wnt pathway is postnatally active and can be further enhanced by Wnt stimulators. Using an in vivo mouse model and RNA sequencing, we show that pro

SUBMITTER: Samarajeewa A 

PROVIDER: S-EPMC6288390 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature