A New Tool for Complement Research: In vitro Reconstituted Human Classical Complement Pathway.

ABSTRACT: The complement, as part of the innate immune system, represents the first line of defense against Gram-negative bacteria invading the bloodstream. The complement system is a zymogen cascade that ultimately assemble into the so-called membrane attack complex (MAC), which lyses Gram-negative bacteria upon insertion into the outer membrane. Traditionally, serum has been used as complement source, for example to study the bactericidal activity of monoclonal antibodies or antibodies raised upon vaccination. Due to the significant donor to donor variability, as well as susceptibility of complement factors to handling and storage conditions, assay reproducibility using human serum is low. Moreover, the presence of pre-existing antibodies and antimicrobial compounds are confounding factors. To rem

SUBMITTER: Mutti M 

PROVIDER: S-EPMC6288441 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature