Perinatal high fat diet and early life methyl donor supplementation alter one carbon metabolism and DNA methylation in the brain.

ABSTRACT: One carbon metabolism is regulated by the availability of nutrients known as methyl donors, and disruption of this pathway can affect multiple physiological systems. DNA methylation, critical for the regulation of gene expression, is linked to one carbon metabolism, and can be altered by perinatal diet. In this study, dams (n = 12/group) were fed HF or standard control (SC) diet through pregnancy and lactation, and male and female offspring were then fed either SC or methyl donor-supplemented diet (MDS) between 3 and 6 weeks of age (n = 20-26/group). Concentration of one carbon intermediates and other related metabolites were assessed within brain tissue (prefrontal cortex, PFC) through the use of mass spectrometry at 6 weeks of age. In addition, the expression of target genes and enzymes

SUBMITTER: McKee SE 

PROVIDER: S-EPMC6288810 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature