Activation of LXRɑ/β by cholesterol in malignant ascites promotes chemoresistance in ovarian cancer.

ABSTRACT:

Background

The purpose of this study was to investigate the role of malignant ascites tumor microenvironment in ovarian cancer progression and chemoresistance.

Methods

A total of 45 patients with ovarian cancer and three benign ascites were collected at the time of clinical intervention. Ascites cholesterol levels were quantitated using cholesterol quantitation kit and recurrence free survival (RFS) of ovarian cancer patients were collected. The sensitivity of ovarian cancer cells to cisplatin (CDDP) and paclitaxel (PAC) were assessed by viability assay, flow cytometry and protein expression. Receiver operating characteristics (ROC) curve and Youden index analysis were applied to calculate the optimal cut-off values for ascites cholesterol. Kaplan-Meier curve were applied to

SUBMITTER: Kim S 

PROVIDER: S-EPMC6288854 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature