Schizont transcriptome variation among clinical isolates and laboratory-adapted clones of the malaria parasite Plasmodium falciparum.

ABSTRACT:

Background

Malaria parasites are genetically polymorphic and phenotypically plastic. In studying transcriptome variation among parasites from different infections, it is challenging to overcome potentially confounding technical and biological variation between samples. We investigate variation in the major human parasite Plasmodium falciparum, generating RNA-seq data on multiple independent replicate sample preparations of merozoite-containing intra-erythrocytic schizonts from a panel of clinical isolates and from long-term laboratory-adapted clones, with a goal of robustly identifying differentially expressed genes.

Results

Analysis of biological sample replicates shows that increased numbers improve the true discovery rate of differentially expressed genes, and that six in

SUBMITTER: Tarr SJ 

PROVIDER: S-EPMC6288915 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature