TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway.

ABSTRACT:

Background

Colorectal cancer (CRC) is one of the most lethal malignancies. Tripartite Motif Containing 14 (TRIM14) is a member of TRIM family proteins, which are involved in the pathogenesis of various cancers. This study aimed to investigate TRIM14 expression in CRC tissues, and its effects on the migration and invasion of CRC cell lines.

Methods

TRIM14 mRNA expression was detected by real-time PCR analysis. Cell migration and invasion were measured by Transwell assays. Protein expression was assessed by western blot analysis.

Results

The expression of TRIM14 was significantly higher in CRC tissues than in matched non-cancerous tissues. TRIM14 knockdown by specific short hairpin RNA (shRNA) attenuated CRC cell migration and invasion, whereas TRIM14 overexpression cau

SUBMITTER: Jin Z 

PROVIDER: S-EPMC6288942 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature