Microtubule Feedback and LET-99-Dependent Control of Pulling Forces Ensure Robust Spindle Position.

ABSTRACT: During asymmetric division of the Caenorhabditis elegans zygote, to properly distribute cell fate determinants, the mitotic spindle is asymmetrically localized by a combination of centering and cortical-pulling microtubule-mediated forces, the dynamics of the latter being regulated by mitotic progression. Here, we show a, to our knowledge, novel and additional regulation of these forces by spindle position itself. For that, we observed the onset of transverse spindle oscillations, which reflects the burst of anaphase pulling forces. After delaying anaphase onset, we found that the position at which the spindle starts to oscillate was unchanged compared to control embryos and uncorrelated to anaphase onset. In mapping the cortical microtubule dynamics, we measured a steep increase in microt

SUBMITTER: Bouvrais H 

PROVIDER: S-EPMC6289040 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature