CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells.

ABSTRACT: To couple the fidelity of patch-clamp recording with a more high-throughput screening capability, we pioneered a, to our knowledge, novel approach to single-channel recording that we named "optical patch clamp." By using highly sensitive fluorescent Ca²⁺ indicator dyes in conjunction with total internal fluorescence microscopy techniques, we monitor Ca²⁺ flux through individual Ca²⁺-permeable channels. This approach provides information about channel gating analogous to patch-clamp recording at a time resolution of ∼2 ms with the additional advantage of being massively parallel, providing simultaneous and independent recording from thousands of channels in the native environment. However, manual analysis of the data generated by this technique presents seve

SUBMITTER: Shah SI 

PROVIDER: S-EPMC6289074 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature