Light-Activated Dynamic Clamp Using iPSC-Derived Cardiomyocytes.

ABSTRACT: iPSC-derived cardiomyocytes (iPSC-CMs) are a potentially advantageous platform for drug screening because they provide a renewable source of human cardiomyocytes. One obstacle to their implementation is their immature electrophysiology, which reduces relevance to adult arrhythmogenesis. To address this, dynamic clamp is used to inject current representing the insufficient potassium current, I_K1, thereby producing more adult-like electrophysiology. However, dynamic clamp requires patch clamp and is therefore low throughput and ill-suited for large-scale drug screening. Here, we use optogenetics to generate such a dynamic-clamp current. The optical dynamic clamp (ODC) uses outward-current-generating opsin, ArchT, to mimic I_K1, resulting in more adult-like action potenti

SUBMITTER: Quach B 

PROVIDER: S-EPMC6289097 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature