Layer-by-layer assembled gold nanoshells for the intracellular delivery of miR-34a.

ABSTRACT:

Introduction

MicroRNAs (miRNAs) are short noncoding RNAs whose ability to regulate the expression of multiple genes makes them potentially exciting tools to treat disease. Unfortunately, miRNAs cannot passively enter cells due to their hydrophilicity and negative charge. Here, we report the development of layer-by-layer assembled nanoshells (LbL-NS) as vehicles for efficient intracellular miRNA delivery. Specifically, we developed LbL-NS to deliver the tumor suppressor miR-34a into triple-negative breast cancer (TNBC) cells, and demonstrate that these constructs can safely and effectively regulate the expression of SIRT1 and Bcl-2, two known targets of miR-34a, to decrease cell proliferation.

Methods

LbL-NS were made by coating negatively charged nanoshells with alternating

SUBMITTER: Goyal R 

PROVIDER: S-EPMC6289203 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature