Veratridine modifies the gating of human voltage-gated sodium channel Nav1.7.

ABSTRACT: Veratridine is a lipid-soluble neurotoxin derived from plants in the family Liliaceae. It has been broadly investigated for its action as a sodium channel agonist. However, the effects of veratridine on subtypes of sodium channels, especially Nav1.7, remain to be studied. Here, we investigated the effects of veratridine on human Nav1.7 ectopically expressed in HEK293A cells and recorded Nav1.7 currents from the cells using whole-cell patch clamp technique. We found that veratridine exerted a dose-dependent inhibitory effect on the peak current of Nav1.7, with the half-maximal inhibition concentration (IC₅₀) of 18.39 µM. Meanwhile, veratridine also elicited tail current (linearly) and sustained current [half-maximal concentration (EC₅₀): 9.53 µM], also in a dose-depend

SUBMITTER: Zhang XY 

PROVIDER: S-EPMC6289357 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature