Raman Spectroscopic Analysis of Signaling Molecules-Dopamine Receptors Interactions in Living Cells.

ABSTRACT: The selective interaction of signaling compounds including neurotransmitters and drugs with the dopamine receptors (DARs) is extremely important for the treatment of neurodegenerative diseases. Here, we report a method to probe the selective interactions of signaling compounds with D1 and D2 DARs in living cells using the combined approach of theoretical calculation and surface-enhanced Raman spectroscopy (SERS). When signaling compounds such as DA, amphetamine, methamphetamine, and methylenedioxypyrovalerone interact with D1 dopamine receptors (DRD1), the intracellular cyclic adenosine monophosphate (cAMP) level is increased. However, the intracellular level of cAMP is decreased when D2 dopamine receptors (DRD2) interact with the abovementioned signaling compounds. In our experiments, we

SUBMITTER: Silwal AP 

PROVIDER: S-EPMC6289496 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature