Modulation of alternative splicing of trafficking genes by genome editing reveals functional consequences in muscle biology.

ABSTRACT: Alternative splicing is a regulatory mechanism by which multiple mRNA isoforms are generated from single genes. Numerous genes that encode membrane trafficking proteins are alternatively spliced. However, there is limited information about the functional consequences that result from these splicing transitions. Here, we developed appropriate tools to study the functional impact of alternative splicing in development within the most in vivo context. Secondly, we provided evidence of the physiological implications of splicing regulation during muscle development. Our previous work in mouse heart development identified three trafficking genes that are regulated by alternative splicing between birth and adulthood: the clathrin heavy chain, the clathrin light chain-a, and the trafficking kinesi

SUBMITTER: Blue RE 

PROVIDER: S-EPMC6289647 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature