High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture.

ABSTRACT: Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell culture models have been used increasingly for in vitro cell culture because they may better model endogenous tissue environments. However, detailed studies of stem cell differentiation within 3D cultures remain limited, particularly with respect to high-throughput screening. Herein, we demonstrate the use of a microarray chip-based platform to screen, in high-throughput, individual and paired effects of 12 soluble factors on the neuronal differentiation of a human neural progenitor

SUBMITTER: Nierode GJ 

PROVIDER: S-EPMC6289706 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature