Association of GRP78 promoter polymorphisms and serum GRP78 level with risk of asthenozoospermia.

ABSTRACT:

Purpose

The aim of this study was undertaken to investigate the association of 78-kDa glucose-regulated protein (GRP78) gene promoter polymorphisms with risk of asthenozoospermia (AZS) men. In addition, we performed association analysis between GRP78 promoter mutations and serum GRP78 level in asthenozoospermia.

Methods

The study population comprised 400 subjects with AZS patients and 400 healthy controls. We assessed GRP78 rs3216733, rs17840761, and rs17840762 polymorphisms by using Snapshot SNP genotyping assays; serum GRP78 level was measured by enzyme-linked immunosorbent assay (ELISA). Semen quality was assessed by computer-assisted semen analysis.

Results

We found that rs3216733 was associated with increased risk of AZS (Gd vs. dd: adjusted OR = 1.42, 95% CI, 1.

SUBMITTER: Qin H 

PROVIDER: S-EPMC6289912 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature