QTL Mapping Combined With Bulked Segregant Analysis Identify SNP Markers Linked to Leaf Shape Traits in Pisum sativum Using SLAF Sequencing.

ABSTRACT: Leaf shape is an important trait that influences the utilization rate of light, and affects quality and yield of pea (Pisum sativum). In the present study, a joint method of high-density genetic mapping using specific locus amplified fragment sequencing (SLAF-seq) and bulked segregant analysis (BSA) was applied to rapidly detect loci with leaf shape traits. A total of 7,146 polymorphic SLAFs containing 12,213 SNP markers were employed to construct a high-density genetic map for pea. We conducted quantitative trait locus (QTL) mapping on an F₂ population to identify QTLs associated with leaf shape traits. Moreover, SLAF-BSA was conducted on the same F₂ population to identify the single nucleotide polymorphism (SNP) markers linked to leaf shape in pea. Two QTLs (

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC6290080 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature