Specific Modification of Aged Proteasomes Revealed by Tag-Exchangeable Knock-In Mice.

ABSTRACT: The proteasome is the proteolytic machinery at the center of regulated intracellular protein degradation and participates in various cellular processes. Maintaining the quality of the proteasome is therefore important for proper cell function. It is unclear, however, how proteasomes change over time and how aged proteasomes are disposed. Here, we show that the proteasome undergoes specific biochemical alterations as it ages. We generated Rpn11-Flag/enhanced green fluorescent protein (EGFP) tag-exchangeable knock-in mice and established a method for selective purification of old proteasomes in terms of their molecular age at the time after synthesis. The half-life of proteasomes in mouse embryonic fibroblasts isolated from these knock-in mice was about 16 h. Using this tool, we found increa

SUBMITTER: Tomita T 

PROVIDER: S-EPMC6290375 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature