CD133+ glioblastoma stem-like cells are radiosensitive with a defective DNA damage response compared with established cell lines.

ABSTRACT:

Purpose

CD133+ glioblastoma tumor stem-like cells (TSC) have been defined as radioresistant. However, although previously classified relative to CD133- cells, the radiosensitivity of CD133+ TSCs with respect to the standard glioblastoma model, established glioma cell lines, has not been determined. Therefore, to better understand the radioresponse of this cancer stem cell, we have used established cell lines as a framework for defining their in vitro radioresponse.

Experimental design

The intrinsic radiosensitivity of CD133+ TSC cultures and established glioma cell lines was determined by clonogenic assay. The TSCs and established cell lines were also compared in terms of DNA double-strand break (DSB) repair capacity and cell cycle checkpoint activation.

Results

Based

SUBMITTER: McCord AM 

PROVIDER: S-EPMC6290462 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature