Transport-exclusion pharmacology to localize lactate dehydrogenase activity within cells.

ABSTRACT:

Background

Recent in vitro and in vivo work has shown that lactate provides an important source of carbon for metabolic reactions in cancer cell mitochondria. An interesting question is whether lactate is oxidized by lactate dehydrogenase (LDH) in the cytosol and/or in mitochondria. Since metabolic processes in the cytosol and mitochondria are affected by redox balance, the location of LDH may have important regulatory implications in cancer metabolism.

Methods

Within most mammalian cells, metabolic processes are physically separated by membrane-bound compartments. Our general understanding of this spatial organization and its role in cellular function, however, suffers from the limited number of techniques to localize enzymatic activities within a cell. Here,

SUBMITTER: Niu X 

PROVIDER: S-EPMC6290536 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature