Role of Electrostatic Interactions on Supramolecular Organization in Calf-Thymus DNA Solutions under Flow.

ABSTRACT: Previous investigations were conducted on two concentrations of DNA solution: 4 mg/mL, for which it has been shown that no supramolecular organization is induced under flow at low shear rates; and 10 mg/mL, in which a liquid crystalline-type texture is formed under flow at low shear rates, attesting to an orientation of pre-organized chains. Rheological experiments are discussed and their results supported by small-angle X-ray scattering (SAXS) and flow birefringence visualization experiments. Scattering from polyelectrolytes has a characteristic signal, which is here observed in SAXS, showing a strong correlation peak between charged chains in water, for both concentrations. This peak is weaker in the presence of 0.01 M NaCl and suppressed in salt excess at 0.1 M NaCl. No plateau in the σ

SUBMITTER: Bravo-Anaya LM 

PROVIDER: S-EPMC6290628 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature