Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma.

ABSTRACT: Microphthalmia-associated transcription factor (MITF) is a key transcription factor in melanoma development and progression. MITF amplification and downregulation have been observed in a significant proportion of melanoma patients and correlate with clinical outcomes. Here, we have investigated the effect of MITF on melanoma chemokine expression and immune cell attraction. In B16F10 melanoma cells, MITF knockdown reduced expression of CXCL10, with concomitantly decreased attraction of immune cells and accelerated tumor outgrowth. Conversely, overexpression of MITF in YUMM1.1 melanoma cells also led to an increased immune cell attraction in vitro. Subcutaneous YUMM1.1 melanomas overexpressing MITF however showed a reduced immune infiltration of lymphocytes and an increased tumor growth. In

SUBMITTER: Wiedemann GM 

PROVIDER: S-EPMC6290759 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature