Deep sequencing of circulating exosomal microRNA allows non-invasive glioblastoma diagnosis.

ABSTRACT: Exosomes are nano-sized extracellular vesicles released by many cells that contain molecules characteristic of their cell of origin, including microRNA. Exosomes released by glioblastoma cross the blood-brain barrier into the peripheral circulation and carry molecular cargo distinct to that of "free-circulating" miRNA. In this pilot study, serum exosomal microRNAs were isolated from glioblastoma (n = 12) patients and analyzed using unbiased deep sequencing. Results were compared to sera from age- and gender-matched healthy controls and to grade II-III (n = 10) glioma patients. Significant differentially expressed microRNAs were identified, and the predictive power of individual and subsets of microRNAs were tested using univariate and multivariate analyses. Additional sera fr

SUBMITTER: Ebrahimkhani S 

PROVIDER: S-EPMC6290767 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature