Basal fatty acid oxidation increases after recurrent low glucose in human primary astrocytes.

ABSTRACT:

Aims/hypothesis

Hypoglycaemia is a major barrier to good glucose control in type 1 diabetes. Frequent hypoglycaemic episodes impair awareness of subsequent hypoglycaemic bouts. Neural changes underpinning awareness of hypoglycaemia are poorly defined and molecular mechanisms by which glial cells contribute to hypoglycaemia sensing and glucose counterregulation require further investigation. The aim of the current study was to examine whether, and by what mechanism, human primary astrocyte (HPA) function was altered by acute and recurrent low glucose (RLG).

Methods

To test whether glia, specifically astrocytes, could detect changes in glucose, we utilised HPA and U373 astrocytoma cells and exposed them to RLG in vitro. This allowed measurement, with high specificity and sensi

SUBMITTER: Weightman Potter PG 

PROVIDER: S-EPMC6290858 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature