Dynamic transcriptome profiling dataset of vaccinia virus obtained from long-read sequencing techniques.

ABSTRACT:

Background

Poxviruses are large DNA viruses that infect humans and animals. Vaccinia virus (VACV) has been applied as a live vaccine for immunization against smallpox, which was eradicated by 1980 as a result of worldwide vaccination. VACV is the prototype of poxviruses in the investigation of the molecular pathogenesis of the virus. Short-read sequencing methods have revolutionized transcriptomics; however, they are not efficient in distinguishing between the RNA isoforms and transcript overlaps. Long-read sequencing (LRS) is much better suited to solve these problems and also allow direct RNA sequencing. Despite the scientific relevance of VACV, no LRS data have been generated for the viral transcriptome to date.

Findings

For the deep characterization of the VACV RNA profi

SUBMITTER: Tombacz D 

PROVIDER: S-EPMC6290886 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature