Experimental challenges regarding the in vitro investigation of the nanoparticle-biocorona in disease states.

ABSTRACT: Toxicological evaluation of nanoparticles (NPs) requires the utilization of in vitro techniques due to their number and diverse properties. Cell culture systems are often lacking in their ability to perform comparative toxicity assessment due to dosimetry issues and capacity to simulate in vivo environments. Upon encountering a physiological environment, NPs become coated with biomolecules forming a biocorona (BC), influencing function, biodistribution, and toxicity. Disease-induced alterations in the biological milieu can alter BC formation. This study evaluates the role of low-density lipoprotein (LDL) in altering macrophage responses to iron oxide (Fe₃O₄) NPs. BCs were formed by incubating Fe₃O₄ NPs in serum-free media, or 10% fetal bovine ser

SUBMITTER: Adamson SX 

PROVIDER: S-EPMC6291017 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature