In vivo Optogenetic Approach to Study Neuron-Oligodendroglia Interactions in Mouse Pups.

ABSTRACT: Optogenetic and pharmacogenetic techniques have been effective to analyze the role of neuronal activity in controlling oligodendroglia lineage cells in behaving juvenile and adult mice. This kind of studies is also of high interest during early postnatal (PN) development since important changes in oligodendroglia dynamics occur during the first two PN weeks. Yet, neuronal manipulation is difficult to implement at an early age because high-level, specific protein expression is less reliable in neonatal mice. Here, we describe a protocol allowing for an optogenetic stimulation of neurons in awake mouse pups with the purpose of investigating the effect of neuronal activity on oligodendroglia dynamics during early PN stages. Since GABAergic interneurons contact oligodendrocyte precursor cells

SUBMITTER: Ortolani D 

PROVIDER: S-EPMC6291523 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature