Differential integrin activity mediated by platelet collagen receptor engagement under flow conditions.

ABSTRACT: The platelet receptors glycoprotein (Gp)VI, integrin α₂β₁ and GpIb/V/IX mediate platelet adhesion and activation during thrombogenesis. Increases of intracellular Ca²⁺ ([Ca²⁺]_i) are key signals during platelet activation; however, their relative importance in coupling different collagen receptors to functional responses under shear conditions remains unclear. To study shear-dependent, receptor-specific platelet responses, we used collagen or combinations of receptor-specific collagen-mimetic peptides as substrates for platelet adhesion and activation in whole human blood under arterial flow conditions and compared real-time and endpoint parameters of thrombus formation alongside [Ca²⁺]_i measurements using confocal

SUBMITTER: Pugh N 

PROVIDER: S-EPMC6291897 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature