Accuracy of RNAseq based SNP discovery and genotyping in Populusnigra.

ABSTRACT:

Backgroud

Populus nigra is a major tree species of ecological and economic importance for which several initiatives have been set up to create genomic resources. In order to access the large number of Single Nucleotide Polymorphisms (SNPs) typically needed to carry out a genome scan, the present study aimed at evaluating RNA sequencing as a tool to discover and type SNPs in genes within natural populations of P. nigra.

Results

We have devised a bioinformatics pipeline to call and type SNPs from RNAseq reads and applied it to P. nigra transcriptomic data. The accuracy of the resulting RNAseq-based SNP calling and typing has been evaluated by (i) comparing their position and alleles to those previously reported in candidate genes, (ii) assessing their genotyping accuracy with

SUBMITTER: Rogier O 

PROVIDER: S-EPMC6291945 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature