Construction of a highly saturated Genetic Map for Vitis by Next-generation Restriction Site-associated DNA Sequencing.

ABSTRACT:

Background

High-saturate molecular linkage maps are an important tool in studies on plant molecular biology and assisted breeding. Development of a large set of single nucleotide polymorphisms (SNPs) via next-generation sequencing (NGS)-based methods, restriction-site associated DNA sequencing (RAD-seq), and the generation of a highly saturated genetic map help improve fine mapping of quantitative trait loci (QTL).

Results

We generated a highly saturated genetic map to identify significant traits in two elite grape cultivars and 176 F₁ plants. In total, 1,426,967 high-quality restriction site-associated DNA tags were detected; 51,365, 23,683, and 70,061 markers were assessed in 19 linkage groups (LGs) for the maternal, paternal, and integrated maps, respectively.

SUBMITTER: Zhu J 

PROVIDER: S-EPMC6291968 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature