The combination of bFGF and CHIR99021 maintains stable self-renewal of mouse adult retinal progenitor cells.

ABSTRACT:

Background

Millions of people are affected with retinal diseases that eventually cause blindness, and retinal progenitor cell (RPC) transplantation is a promising therapeutic avenue. However, RPC expansion and the underlying regulation mechanisms remain elusive.

Methods

Adult mouse neural RPCs (mNRPCs) were isolated and amplified with the combination of basic fibroblast growth factor (bFGF) and glycogen synthase kinase 3 (GSK3) inhibitor CHIR99021. The progenitor characteristics were evaluated with RT-PCR, immunocytochemistry (ICC), western blot, flow cytometry, and transcriptome analysis prior to transplantation. By treating cells with or without bFGF and CHIR99021 at different time points, the mechanism for mNRPCs' self-renewal was investigated by transcriptome analysis an

SUBMITTER: Jin C 

PROVIDER: S-EPMC6292077 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature