Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients.

ABSTRACT:

Background

Demineralized dentin matrix (DDM) is commonly used as a bone-graft substitute. This study measured and compared human hepatitis B viruses (HBV) DNA in fresh dentin to that of dentin processed into DDM extracted during dental treatment from HBV-infected patients. The hypothesis was that the processing procedure for DDM would inactivate or eliminate HBV in the dentin matrix obtained from infected patients.

Methods

Dentin from eighteen HBV-infected patients was collected and each dentin specimen was divided into two fragments. One fragment was used before processing as fresh dentin (control group) and the other was processed into DDM (experimental group). DNA was extracted and purified from each fresh and processed dentin specimen and the HBV DNA copy number quantita

SUBMITTER: Um I 

PROVIDER: S-EPMC6292124 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature