Analysis of 3D genomic interactions identifies candidate host genes that transposable elements potentially regulate.

ABSTRACT:

Background

The organization of chromatin in the nucleus plays an essential role in gene regulation. About half of the mammalian genome comprises transposable elements. Given their repetitive nature, reads associated with these elements are generally discarded or randomly distributed among elements of the same type in genome-wide analyses. Thus, it is challenging to identify the activities and properties of individual transposons. As a result, we only have a partial understanding of how transposons contribute to chromatin folding and how they impact gene regulation.

Results

Using PCR and Capture-based chromosome conformation capture (3C) approaches, collectively called 4Tran, we take advantage of the repetitive nature of transposons to capture interactions from multiple copie

SUBMITTER: Raviram R 

PROVIDER: S-EPMC6292174 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature