Allele Phasing Greatly Improves the Phylogenetic Utility of Ultraconserved Elements.

ABSTRACT: Advances in high-throughput sequencing techniques now allow relatively easy and affordable sequencing of large portions of the genome, even for nonmodel organisms. Many phylogenetic studies reduce costs by focusing their sequencing efforts on a selected set of targeted loci, commonly enriched using sequence capture. The advantage of this approach is that it recovers a consistent set of loci, each with high sequencing depth, which leads to more confidence in the assembly of target sequences. High sequencing depth can also be used to identify phylogenetically informative allelic variation within sequenced individuals, but allele sequences are infrequently assembled in phylogenetic studies. Instead, many scientists perform their phylogenetic analyses using contig sequences which result from t

SUBMITTER: Andermann T 

PROVIDER: S-EPMC6292485 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature