A yellow fever-Zika chimeric virus vaccine candidate protects against Zika infection and congenital malformations in mice.

ABSTRACT: The recent Zika virus (ZIKV) epidemic in the Americas led to an intense search for therapeutics and vaccines. Here we report the engineering of a chimeric virus vaccine candidate (YF-ZIKprM/E) by replacing the antigenic surface glycoproteins and the capsid anchor of YFV-17D with those of a prototypic Asian lineage ZIKV isolate. By intracellular passaging, a variant with adaptive mutations in the E protein was obtained. Unlike YFV-17D, YF-ZIKprM/E replicates poorly in mosquito cells. Also, YF-ZIKprM/E does not cause disease nor mortality in interferon α/β, and γ receptor KO AG129 mice nor following intracranial inoculation of BALB/c pups. A single dose as low as 1 × 10² PFU results, as early as 7 days post vaccination, in seroconversion to neutralizing antibodies and confers full

SUBMITTER: Kum DB 

PROVIDER: S-EPMC6292895 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature