Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography.

ABSTRACT: Combining tomography with electron microscopy (EM) produces images at definition sufficient to visualize individual protein molecules or molecular complexes in intact neurons. When freeze-substituted hippocampal cultures in plastic sections are imaged by EM tomography, detailed structures emerging from 3D reconstructions reveal putative glutamate receptors and membrane-associated filaments containing scaffolding proteins such as postsynaptic density (PSD)-95 family proteins based on their size, shape, and known distributions. In limited instances, structures can be identified with enhanced immuno-Nanogold labeling after light fixation and subsequent freeze-substitution. Molecular identification of structure can be corroborated in their absence after acute protein knockdown or gene knockout

SUBMITTER: Chen X 

PROVIDER: S-EPMC6292990 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature