Intracellular localization of polymyxins in human alveolar epithelial cells.

ABSTRACT:

Background

Current inhaled polymyxin therapy is empirical and often large doses are administered, which can lead to pulmonary adverse effects. There is a dearth of information on the mechanisms of polymyxin-induced lung toxicity and their intracellular localization in lung epithelial cells.

Objectives

To investigate the intracellular localization of polymyxins in human lung epithelial A549 cells.

Methods

A549 cells were treated with polymyxin B and intracellular organelles (early and late endosomes, endoplasmic reticulum, mitochondria, lysosomes and autophagosomes), ubiquitin protein and polymyxin B were visualized using immunostaining and confocal microscopy. Fluorescence intensities of the organelles and polymyxin B were quantified and correlated for co-localization

SUBMITTER: Ahmed MU 

PROVIDER: S-EPMC6293086 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature