Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS.

ABSTRACT: Spinal motor axons traverse large distances to innervate target muscles, thus requiring local control of cellular events for proper functioning. To interrogate axon-specific processes we developed Axon-seq, a refined method incorporating microfluidics, RNA sequencing (RNA-seq), and bioinformatic quality control. We show that the axonal transcriptome is distinct from that of somas and contains fewer genes. We identified 3,500-5,000 transcripts in mouse and human stem cell-derived spinal motor axons, most of which are required for oxidative energy production and ribogenesis. Axons contained transcription factor mRNAs, e.g., Ybx1, with implications for local functions. As motor axons degenerate in amyotrophic lateral sclerosis (ALS), we investigated their response to the SOD1^G93A m

SUBMITTER: Nijssen J 

PROVIDER: S-EPMC6294264 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature