UM-HACC-2A: MYB-NFIB fusion-positive human adenoid cystic carcinoma cell line.

ABSTRACT:

Objectives

Limited availability of validated human adenoid cystic carcinoma (ACC) cell lines has hindered the mechanistic understanding of the pathobiology of this malignancy and the development of effective therapies. The purpose of this work was to generate and characterize a human ACC cell line.

Material and methods

Immediately after surgery, a tumor fragment from a minor salivary gland from the tongue of a female Caucasian was minced, dissociated, and a single cell suspension was plated in fibronectin-coated flasks. A culture medium containing bovine brain extract and rhEGF was optimized for these cells. Whole exome sequencing was used to evaluate the presence of MYB-NFIB translocation.

Results

The University of Michigan-Human Adenoid Cystic Carcinoma (UM-HACC)-2A

SUBMITTER: Warner KA 

PROVIDER: S-EPMC6294471 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature