Decomposition of time-dependent fluorescence signals reveals codon-specific kinetics of protein synthesis.

ABSTRACT: During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted by an individual peptide is determined by co-translational events, such as secondary structure formation and peptide-tunnel interactions. To obtain information on these individual events, the measured ensemble fluorescence signal has to be decomposed into position-dependent intensities. Here, we describe mRNA translation as a Markov process with specific fluorescence intensities assigned to the different states of the process. Combining the computed stochastic time evolution o

SUBMITTER: Haase N 

PROVIDER: S-EPMC6294494 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature