Longitudinal Biochemical Assay Analysis of Mutant Huntingtin Exon 1 Protein in R6/2 Mice.

ABSTRACT:

Background

Biochemical analysis of mutant huntingtin (mHTT) aggregation species in HD mice is a common measure to track disease. A longitudinal and systematic study of how tissue processing affects detection of conformers has not yet been reported. Understanding the homeostatic flux of mHTT over time and under different processing conditions would aid in interpretation of pre-clinical assessments of disease interventions.

Objective

Provide a systematic evaluation of tissue lysis methods and molecular and biochemical assays in parallel with behavioral readouts in R6/2 mice to establish a baseline for HTT exon1 protein accumulation.

Methods

Established biochemical methods were used to process tissue from R6/2 mice of specific ages following behavior tasks. Aggregation s

SUBMITTER: Morozko EL 

PROVIDER: S-EPMC6294605 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature