Intracellular emetic signaling cascades by which the selective neurokinin type 1 receptor (NK₁R) agonist GR73632 evokes vomiting in the least shrew (Cryptotis parva).

ABSTRACT: To characterize mechanisms involved in neurokinin type 1 receptor (NK₁R)-mediated emesis, we investigated the brainstem emetic signaling pathways following treating least shrews with the selective NK₁R agonist GR73632. In addition to episodes of vomiting over a 30-min observation period, a significant increase in substance P-immunoreactivity in the emetic brainstem dorsal motor nucleus of the vagus (DMNX) occurred at 15 min post an intraperitoneal (i.p.) injection GR73632 (5 mg/kg). In addition, time-dependent upregulation of phosphorylation of several emesis -associated protein kinases occurred in the brainstem. In fact, Western blots demonstrated significant phosphorylations of Ca²⁺/calmodulin kinase IIα (CaMKIIα), extracellular signal-regulated protein k

SUBMITTER: Zhong W 

PROVIDER: S-EPMC6294657 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature