Derivation and characterization of putative craniofacial mesenchymal progenitor cells from human induced pluripotent stem cells.

ABSTRACT: The introduction and widespread adoption of induced pluripotent stem cell (iPSC) technology has opened new avenues for craniofacial regenerative medicine. Neural crest cells (NCCs) are the precursor population to many craniofacial structures, including dental and periodontal structures, and iPSC-derived NCCs may, in the near future, offer an unlimited supply of patient-specific cells for craniofacial repair interventions. Here, we used an established protocol involving simultaneous Wnt signaling activation and TGF-β signaling inhibition to differentiate three human iPSC lines to cranial NCCs. We then derived a mesenchymal progenitor cell (NCC-MPCs) population with chondrogenic and osteogenic potential from cranial NCCs and investigated their similarity to widely studied human postnatal den

SUBMITTER: Jamal M 

PROVIDER: S-EPMC6294687 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature