Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility.

ABSTRACT: Drug resistance is an obstacle to global malaria control, as evidenced by the recent emergence and rapid spread of delayed artemisinin (ART) clearance by mutant forms of the PfKelch13 protein in Southeast Asia. Identifying genetic determinants of ART resistance in African-derived parasites is important for surveillance and for understanding the mechanism of resistance. In this study, we carried out long-term in vitro selection of two recently isolated West African parasites (from Pikine and Thiès, Senegal) with increasing concentrations of dihydroartemisinin (DHA), the biologically active form of ART, over a 4-y period. We isolated two parasite clones, one from each original isolate, that exhibited enhanced survival to DHA in the ring-stage survival assay. Whole-genome sequence anal

SUBMITTER: Demas AR 

PROVIDER: S-EPMC6294886 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature