NETSeq reveals heterogeneous nucleotide incorporation by RNA polymerase I.

ABSTRACT: DNA sequence motifs that affect RNA polymerase transcription elongation are well studied in prokaryotic organisms and contribute directly to regulation of gene expression. Despite significant work on the regulation of eukaryotic transcription, the effect of DNA template sequence on RNA polymerase I (Pol I) transcription elongation remains unknown. In this study, we examined the effects of DNA sequence motifs on Pol I transcription elongation kinetics in vitro and in vivo. Specifically, we characterized how the spy rho-independent terminator motif from Escherichia coli directly affects Saccharomyces cerevisiae Pol I activity, demonstrating evolutionary conservation of sequence-specific effects on transcription. The insight gained from this analysis led to the identifica

SUBMITTER: Clarke AM 

PROVIDER: S-EPMC6294894 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature