Mechanism of the E2 to E1 transition in Ca²⁺ pump revealed by crystal structures of gating residue mutants.

ABSTRACT: Ca²⁺-ATPase of sarcoplasmic reticulum (SERCA1a) pumps two Ca²⁺ per ATP hydrolyzed from the cytoplasm and two or three protons in the opposite direction. In the E2 state, after transferring Ca²⁺ into the lumen of sarcoplasmic reticulum, all of the acidic residues that coordinate Ca²⁺ are thought to be protonated, including the gating residue Glu309. Therefore a Glu309Gln substitution is not expected to significantly perturb the structure. Here we report crystal structures of the Glu309Gln and Glu309Ala mutants of SERCA1a under E2 conditions. The Glu309Gln mutant exhibits, unexpectedly, large structural rearrangements in both the cytoplasmic and transmembrane domains, apparently uncoupling them. However, the structure definitely represents E2 and,

SUBMITTER: Tsunekawa N 

PROVIDER: S-EPMC6294896 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature