Cancer-associated fibroblasts suppress SOX2-induced dysplasia in a lung squamous cancer coculture.

ABSTRACT: Tumorigenesis depends on intricate interactions between genetically altered tumor cells and their surrounding microenvironment. While oncogenic drivers in lung squamous carcinoma (LUSC) have been described, the role of stroma in modulating tissue architecture, particularly cell polarity, remains unclear. Here, we report the establishment of a 3D coculture system of LUSC epithelial cells with cancer-associated fibroblasts (CAFs) and extracellular matrix that together capture key components of the tumor microenvironment (TME). Single LUSC epithelial cells develop into acinar-like structures with 0.02% efficiency, and addition of CAFs provides proper tumor-stromal interactions within an appropriate 3D architectural context. Using this model, we recapitulate key pathological changes during tum

SUBMITTER: Chen S 

PROVIDER: S-EPMC6294935 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature